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KMID : 1007520010100030251
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Food Science and Biotechnology
2001 Volume.10 No. 3 p.251 ~ p.256
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Inhibition of Hepatic Glucose-6-Phosphatase by Solvent Extracts of Prosomillet
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LEE, HYE KYUNG
Cho, Sung Hee/Choi, Young Sun/Chang, Eun Ju/Choi, Sang Won/Ha, Tae Youl
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Abstract
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Hepatic glucose-6-phosphatase (G-6-Pase) (EC 3.1.3.9) liberating glucose from glucose-6-phosphate (G6-P) is recognized to be a significant factor causing abnormally high rates of glucose production in the diabetic liver. Inhibitory activity on rat liver microsomal G-6-Pase activity was examined in solvent extracts of prosomillet (Panicum milaceum L.). Inhibition by methnanol extract of prosomillet was almost 60% at the concentration of 0.2 ㎍/㎍ micorosomal protein. When second solvent extractions were used, the inhibitory activities were detected in the hexane and chloroform extracts but not in the buthanol and ethylacetate ones. The hexane fraction showing the inhibitory activity was further separated through silicic acid column chromatography by eluting the mixture of hexane and ethylacetate (8:1, v/v). The eluted fractions were partitioned into seven groups according to their chemical similarities detected through thin layer chromatography, and the seven combined fractions were crystalized. The G-6-Pase inhibitory activities were found in five combined fractions with Rf values of 0.25-0.5. The degree of inhibition was approximately 70% at the level of 0.04 ㎍ crystals/㎍ micorosomal protein and increased further at higher levels. Enzyme inhibitons by 1.4 mM 2-hydroxy-5-nitro-benzaldehyde and 9 mM pyridoxal 5-phosphate were not blocked by any fraction with inhibitory activity. When prosomillet powder was fed to rats at the level of 30% (w/w), fasting plasma glucose was significantly reduced, but liver G-6-Pase activity was not changed. The present results suggest that the inhibitory substances in prosomillet have the potential to alleviate hyperglycemia in diabetes and other physiological complications related to G-6-Pase activity.
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